Biocontrol agent of root-knot nematode Meloidogyne javanica and root-rot fungi, Fusarium solani in okra morphological, anatomical characteristics and productivity under greenhouse conditions

This study was conducted to evaluate the ability of some fungal culture filtrate, as biocontrol agents against okra wilt caused by Fusarium solani. and Meloidogyne javanica. In the present study, fungal culture filtrates (FCFs) of Aspergillus terreus (1), Aspergillus terreus (2), Penicillium chrysogenum, and Trichoderma spp. were tested against M. javanica in vitro. The effects of P. chrysogenum and Trichoderma spp. (FCFs) in controlling root-rot fungi and root-knot nematode disease complex on okra plants were studied under greenhouse conditions (In vivo). In vitro experiment, the results revealed cumulative rate of J2s mortality of M. javanica reached to 97.67 and 95% by P. chrysogenum and Trichoderma spp., respectively, after 72 h. incubation. Additionally, Trichoderma spp exhibited the most effective inhibitory activity against the pathogen's radial growth, with a percentage of 68%. P. chrysogenum ranked second with 53.88%, while A. terreus (2) demonstrated the weakest inhibitory effect of 24.11%. T6 [Nematode infection (M. javanica) + Fungus infection (F. solani) + Overflowed with fungal culture filtrate (P. chrysogenum)] and T8 [Nematode infection (M. javanica) + Fungus infection (F. solani) + spray with fungal culture filtrate (P. chrysogenum)] had the greatest effects on nematode galling indices on okra roots and substantially reduced the reproductive factors in the greenhouse (In vivo experiment). T6 was the best treatment to decrease disease severity, as reached (28%) relatively. On the other hand, T12 [(Fungus infection (F. solani) + (Dovex 50% fungicide with irrigation water)] recorded the lowest disease severity reaching (8%) relatively. The results showed that nematode infection or fungus infection or both decreased all studied anatomical characteristics of okra root, stem, and leaves. We concluded from this study that root-knot nematode and root-rot fungi were reduced by using fungal culture filtrates and could improve plant growth.

www.nature.com/scientificreports/ Plant-parasitic nematodes are among the most damaging pests of vegetable crops, accounting for significant global agricultural production losses each year 3 . Root-knot nematodes are the most common and commercially significant nematode pests, with a life cycle of 25 days at 27 °C 4 . Nematode and fungal infestations are considered the most important biotic factors that lower crop production, and nematodes can also predispose plants to secondary invasion by fungal diseases, exacerbating disease severity by modifying plant roots 5 .
Chemical control of nematodes and fungi is costly and has potential environmental and public health risks 6,7 . Therefore, developing safe and effective techniques for managing these harmful infections is essential. Biological control is a safer alternative to chemical nematicides, and Penicillium and Trichoderma fungi have a reputation for being hostile to plant-parasitic nematodes 8,9 . However, little is known about the mode of action of fungal culture filtrates (FCF) and their efficacy in controlling complex diseases caused by root-knot nematodes and rot-root fungi in okra plants.
In this study, we investigate the impact of biological control agents' strategy (fungal culture filtrates) in the control of the complex disease caused by the root-knot nematode and rot-root fungi. We also examine the mode of action of FCF on the morphological traits, anatomical analysis, and productivity of okra plants. Our study aims to contribute to the development of safe and effective techniques for the prevention and control of pathogenic agents in okra production.

Materials and methods
Isolation and identification of the causal pathogens. On Potato Dextrose Agar (PDA) medium, the causative pathogenic fungi were isolated using the agar plate technique 10 . Using the conventional hyphal tip approach, Fusarium spp. was isolated from the diseased Okra plants and introduced into pure culture 11 . To stop bacterial growth in the medium before sterilization, 250 mg L −1 of chloramphenicol (l-chloramphenicol) was added 12 . Utilizing either the single spore or hyphal tip procedures, the produced fungus were purified 13 . For subsequent studies, stock cultures were kept on PDA slants in a refrigerator at 5 °C. The physical characteristics of mycelia and spores, as described by Leslie and Summerell 14 and Ismail,et al. 15 were used to identify fungal isolates.
Isolation and identification of endophytic fungi. The modified approach developed by Hallmann, et al. 16 was used to isolate endophytic fungi. The obtained root and branch samples were carefully cleaned with sterile distilled water after being washed with mild detergent and running tap water to get rid of the dirt particles and adherent debris. For one minute, 70% ethanol was used to surface sterilize the samples. The samples were submerged in 4% sodium hypochlorite for 3 min to thoroughly sterilize the surfaces and get rid of any clinging germs. The samples were then dried on sterile filter paper after being washed with sterile distilled water. Using a sterile scalpel, the samples were divided into 5-10 mm pieces and grown in Petri plates with PDA media supplemented with chloramphenicol (250 mg L -1 ) to inhibit bacterial growth. Petri plates were covered with parafilm, incubated for 15 days at 27 ± 2 °C in the dark, and daily checked. Separate transfers of the purified endophytic fungal isolates to PDA slants were made. Finally, all the purified endophytic fungi were stored at 4 °C until further use. According to Domsch et al. 17 , Domsch et al. 18 , endophytic fungus isolates were recognized at the Assiut University Moubasher Mycological Center (AUMMC), Assiut, Egypt based on their macro-and microscopic features. Endophytic fungi P. chrysogenum and Trichoderma spp. were grown on potato dextrose broth (PDB) and incubated at 25 °C for 21 days, then filtered through sterilized cheesecloth to remove mycelium mats, then filtered through Membrane Solutions MS CA Syringe Filter (0.45 μm) to obtain cell-free culture filtrates then kept in freezer (at 5 °C) as stander solutions until use.

Nematode inoculums.
A pure culture of M. javanica was grown from a single egg mass removed from infected okra roots and kept alive on tomato cv. Strian-B in greenhouse. The whole root system of infected plants was dipped in water and carefully cleaned to eliminate any clinging dirt before being uprooted from the soil. Forceps were used to remove egg masses, which were subsequently cleaned with sterile water, put in a 0.5% sodium hypochlorite (NaOCl) solution, stirred for four minutes, and then dried on a 26 µm sieve 19 . A modified version of Baermann funnel technique was used to incubate the eggs for 3-5 days in order to produce secondstage juveniles (J 2 ) for in vitro and pot studies 20 . In vitro experiment. Evaluation of fungal culture filtrates on M. javanica mortality. The assessment was done in 5 cm clean Petri dishes. As a control, Petri plates filled with distilled water were used. A. terreus (1), A. terreus (2), P. chrysogenum, and Trichoderma spp. fungal culture filtrates of concentration S/5 were prepared by adding 5 mL of distilled water to 1 mL of standard concentration "S" to study the juvenile's mortality. One hundred from second stage (J 2 ) of M. javanica were used in the study. Three replications of each treatment were used. All dead and alive (J 2 ) were counted after 72 h of incubation to determine the proportion of J 2 mortality.
Evaluation of fungal culture filtrates on F. solani radial colony growth. The study aimed to evaluate the effectiveness of culture filtrates derived from endophytic fungi in controlling F. solani. To achieve this, sterilized Petri dishes were filled with culture filtrates of A. terreus (1), A. terreus (2), P. chrysogenum, and Trichoderma spp., all at a 10% concentration (v/v). The PDA medium was allowed to solidify before placing 6 mm diameter mycelia discs of F. solani obtained from actively growing colonies in the center of the solidified agar plates. The control group was treated with sterilized distilled water instead of culture filtrate. The Petri dishes were then incubated at 25 ± 2 °C for four days, after which the percentage of inhibition in radial colony growth was calculated by comparing the results with the control group. The percent inhibition of mycelia growth of the pathogens was calculated using the following formula 21 .
Experimental design and treatments. The  Fungal culture filtrates (FCFs) were applied to plants as overflowed or sprayed onto leaf surface four days after inoculation by nematode (two weeks after planting). Oxamyl 24% and Dovex 50% were applied in the dose recommended by the manufacturer for resistance to nematodes and Fungus, respectively.
During the experiment, crop measurements (the number of fruits, fruit length, and fruit weight) every 3 days were estimated. After 8 weeks after inoculation, the plants were carefully uprooted from the soil and measured for shoot length, root length, plant weight, stem diameter, number of branches, leaf area, and leaf number. Additionally, the number of galls, egg-masses/root, and eggs/egg-mass were counted. The ultimate nematode population and nematode reproduction rate were estimated.
According to Abdel-Razik, et al. 22 , the Fusarium wilt disease severity (%) was determined after 60 days had passed since planting using the disease severity (DS) scale (0:5), which indicated [0 = no visible symptoms; 1 = slight vein-clearing and chlorosis of the leaves; 2 = yellowing and wilting of lower leaves and extend to upper leaves; 3 = brown (discoloration) of the vascular systems of tap rot and stem; 4 = indicates necrotic streaks on the stem base that extend toward the apex, while 5 = indicates an early plant demise. The following equation was used to calculate the percentage of disease severity index (DSI) for each tested isolate: where (d) is the overall disease rating for each plant, (d max) is the maximum disease rating, (n) is the total number of plants tested in each duplicate.
Anatomical study. At 60 days following planting, a comparative anatomical investigation of the root, stem, and leaf of treated and control okra plants was conducted. Okra root, stem, and terminal leaf samples were gathered. The samples of stem and leaf were obtained from the 5th apical internode of the main stem and its corresponding leaf with the most successful treatments, i.e., T1, T5, T6, T7, and T13, in addition to those of the control (T14) after 60 days. The root tips were sampled at a distance of 0.5 cm. The samples were collected, killed, and preserved in a solution containing (5 mL formalin, 10 mL glacial acetic acid, and 85 mL 70% ethanol). It was then washed in 50% ethyl alcohol, dehydrated in a succession of ethyl alcohols (50,70,80,90, 95, and 100%), infiltrated in xylene, embedded in paraffin wax with a melting temperature of 40-45 °C, sectioned on a rotary microtome at a thickness of 5-7 m, dyed with the double stain procedure (light green and safranin),  23 . Sections were examined to look for histological evidence of treatment-related noticeable responses. The prepared sections were examined under a microscope and counts and measurements (µ) were taken using computerized morphometrical analysis. This analysis was carried out using a Research Microscope type Axiostar plus manufactured by Zeiss that was upgraded to be able to use a professional digital image analysis system (Carl Zeiss Axiovision Product Suite DVD 30).
Statistical analysis. Three to four duplicate experiments were run for each treatment to satisfy the random design. One-way analysis of variance (ANOVA), which was performed using the software (Statistix 8.1) for statistical analysis of the experiment's results, was used to analyze the growth parameters and yield and assess for significance. To look at the variations between the means that were statistically significant, Duncan's multiple range tests with 95% confidence were performed 24 . Statement permission. The authors declare that they have proper permission.

Results
In vitro experiment. Effect of FCFs on root-knot nematode. The fungal culture filtrates (FCFs) of A. terreus (1), A. terreus (2), P. chrysogenum and Trichoderma spp. were tested for their nematicidal effect on second stage juveniles of M. javanica in vitro (Fig. 1). The results revealed that the nematode mortalities with fungal culture filtrates were increased with the increase of the exposure time from 24 to 72 h (FCF) of Trichoderma spp. was more effective against M. javanica J 2 followed by P. chrysogenum with mortality rates (97.67 & 95%), respectively, after 72 h of exposure time.
Effect of FCFs on F. solani radial colony growth. An experiment was conducted in a laboratory to investigate the efficacy of fungal culture filtrates (FCFs) derived from endophytic fungi at a 10% concentration (v/v) as biocontrol agents against F. solani. The study involved testing the FCFs of A. terreus (1) and A. terreus (2), P. chrysogenum, and Trichoderma spp. against the causal pathogen (as shown in Figs. 2 and 3A-E). The results revealed that the FCFs inhibited the growth of the pathogen with varying degrees of effectiveness. Treatment with FCFs of these antagonistic fungi significantly reduced the linear growth of the tested pathogen compared to the control (distilled water). Notably, Trichoderma spp ( Fig. 3D) showed the highest percent inhibition of radial growth of the causal pathogen (68%), followed by P. chrysogenum (53.88%) (Fig. 3C). In contrast, A. terreus (2) had the lowest inhibitory effect (24.11%) (Fig. 3B).

Greenhouse experiment. Effect of fungal culture filtrates (FCFs) on root-rot fungus and root-knot nematode
under greenhouse conditions. The effects of P. chrysogenum (FCF P ) and Trichoderma spp. (FCF T ) applied as foliar spray and soil drenches on control of root-rot fungus (F. solani) and root-knot nematode (M. javanica) disease complex on growth and anatomical characteristics of okra plants cv. OH-102 were studied under greenhouse conditions. Data presented in Table 1 and Fig. 4 showed that, all applications of the tested (FCFs) significantly reduced the values of root galls per root and nematode reproduction on roots of okra when compared with untreated plants [control (T14). Therefore, (FCF P ) as soil drenches (T4) and foliar sprays (T8) caused the lowest gall numbers (40 and 49)], respectively. While (FCF P ) as soil drenches (T4) and foliar sprays (T8) caused the lowest rate of nematode reproductions ( Table 1 indicate that F. solani was able to infect okra plants causing root rot with varied degrees. Therefore, T5 caused the highest disease severity (60%) followed by T1 and T11 (48%). While the disease severity of T3 and T9 observed the same disease severity (40%) relatively. On the other hand, T6 were recorded the lowest disease severity as reached (28%) relatively followed by T12 (8%).
Morphological characteristics. Plant metrics were offered lowest significant, under the circumstances of single nematode infection, single fungal infection, or both of them together (Table 2). Generally, application of the fungal culture filtrates (FCF P, T ) resulted in a significant increase in most of the measured traits, whether it was a spraying system or overflowed, the tallest shoot recorded by T12 and T8 were (42.   In this study, the (FCF T ) as a foliar spray for nematode and fungal infection combined (T9) equal in the number of fruits with control treatment (T14). The greatest increase of fruit length was under T4 (9.93 cm) followed by (FCF P ) as overflowed with treated nematode and fungal infection together and fungal infection T6 (9.77 cm), as well the lowest value was reported by nematode and fungal infection treatment T5 (7.93 cm). Application of the (FCF P ) as overflowed with nematode and fungal infection combined (T6) achieved the weight of the fruits (11.13 g), followed by (FCF T ) treat as a foliar spray with nematode and fungal infection combined T9 (10.43 g), while the lowest values noted by nematode and fungal infection treatment T5 (7.17 g).
Anatomical studies. The Anatomical studies to follow up the internal structure changes, which exhibited the most noticeable response against root-knot nematode, root-rot fungi, and fungal culture filtrate of P. chrysogenum applied as overflowed or foliar spray and their effect on the mean counts and measurements in micron (μ) of certain histological features of root, stem and leaf of okra grown in the plastic pots at 60 days after sowing based on transverse sections. Data in Table 3 Table 3 and Fig. 5. Numerous anatomical characteristics, particularly the T5, saw obvious reductions in their thickness. Since it had the www.nature.com/scientificreports/ most adverse impact on the anatomical traits that had been evaluated. The most significant anatomical characteristics were i.e., Ø of root (µm), Ø of V.C(µm), and Xylem arm length (µm), where all of them were significantly reduced with T5 compared with control (T14) and T7. It's interesting to notice that the treatments' good effects on many anatomical characteristics were entirely undone when the treated plants' vegetative and reproductive development was increased, particularly in the cases of the control (T14) and (T7) treatments when compared to other treatments. The current investigation demonstrated increases in both xylem tissue, which is responsible for transporting various assimilates from leaves to fruits and other plant sinks, and phloem tissue, which is responsible for transporting water and mineral nutrients from roots to leaves. Therefore, the increase in the ultimate fruit yield might be directly attributed to the improvement of translocation events.    Table 3 and Fig. 6 show that, the treatments of nematode infection (T1) or fungi infection (T13) or nematode infection + fungus infection (T5) in the present study decreased the most of okra stem anatomical features compared with the (T14), T6, and T7. Okra plants with control (T14) and T7 appeared to be the most effective treatment. This treatment manifested the best results as it exceeded that of nematode infection + fungus infection (T5) in terms of most studied anatomical features. With regard to the stem diameter, it was reached its maximum value (3807.31 eμ) with nematode infection + fungus infection (T5), comparing with other treatments. Application treatments of control (T14) and T7 recorded highly values of the stem studied anatomical characteristics especially, in the Ø of root (µm), Ø of V.C (µm)and xylem arm length (µm) compared with other applied treatments. It's interesting to notice that improving the vegetative and reproductive development of treated plants fully reversed these beneficial responses of various anatomical characteristics to treatments, particularly in the case of control (T14) and T7 compared to other treatments. The current investigation therefore demonstrated such expansions of xylem tissue, or the conduit of various assimilates from leaves to fruits and other plant sinks, and phloem tissue, or the route of water and mineral nutrient translocation from roots to leaves. As a result, an increase in the ultimate fruit production might be directly attributed to better translocation occurrences.
Leaf anatomy. The information in Table 3 and Fig. 7 demonstrated how the examined histological characteristics of the leaf reacted similarly to the anatomical characteristics of the stem. For mesophyll tissue, the application of M. javanica (T1), F. solani (T13), and M. javanica + F. solani had a significant impact on the thickness of both the spongy and palisade tissues (T5). Here, spongy tissue thickness value was decreased (127.85 cμ) with T5 but increased to reach (133.29 cμ) and (128.53 cμ) with T1 and T13, respectively, which were the more effective treatments in the same order. By contrast, palisade tissue thickness was (143.51 d and 142.64 dμ) with T1 and T13, respectively, but decreased to reach (141.79 dμ) with T5. With regard to V.B. thickness (µm) was reached their maximum values with T5 treatment. Here, V.B. thickness (µm) was decreased (170.67 fμ) with T5 while increased to reach (193.14 d) and (185.81 eμ) with T1 and T13, respectively, which were the more effective treatments in the same order.
The acquired results generally show that administered therapies, particularly T6 and T7, play a protective defensive function as resistance-inducers against detrimental effects by improving anatomical performance and metabolic defense system. The data indicated that, clearly indicate that applied treatments especially, control (T14), T6 and T7 play a defensive protective role as resistance -inducers against adverse effects by enhancing the anatomical performance and metabolically defense system had a positive effect on all studied anatomical characteristics of root (Ø of root, cortex thickness, Ø of V.C and length of xylem arch), for stem (Ø of stem, cortex thickness, Ø of V.C and V.B thickness) as well as leaves (spongy and palisade tissues thickness and V.B thickness). While the used treatments of T1 and T13 had a negative effect on most of the studied anatomical characteristics

Discussion
The biocontrol potential of P. chrysogenum and Trichoderma spp. against root knot nematodes and Fusarium spp. was further confirmed by both laboratory and pot trial results. Trichoderma spp. species have been reported to suppress Fusarium spp. and plant parasitic nematodes [25][26][27] due to their ability to produce hydrolytic enzymes that break down chitin in fungal cell walls 28 as well as antibiotics 29 . Trichoderma spp. can directly control root-knot nematodes by producing poisonous compounds that prevent their penetration and growth [30][31][32] . For instance, T. viride and T. harzianum reduced the population of M. javanica in okra 33,34 and tomato plants [35][36][37] . Their destructive activities include enzymatic disruption of eggshells and larval cuticles, as well as physiological abnormalities resulting from the production of diffusible toxic metabolites 38,39 . Studies by Sankari Meena, et al. 40 and Poveda,et al. 41 reported that the use of T. harzianum resulted in improved plant development metrics and a reduction in the fungal-nematode disease complex. Additionally, T. harzianum has been found to induce plant resistance and promote plant growth 42 . Nematotoxic properties of diverse fungi's fungal culture filtrates add a new level of phytonematode biocontrol. Fungal culture filtrate of T. harzianum has been shown to have nematotoxic effects 43 , Alternaria alternata, A. flavus, P. chrysogenum, Rhizoctonia bataticola, T. viride have been recorded by Kumar, et al. 44 .
By decreasing the pathogen's development by up to 60.4%, the culture filtrate from T. harzianum displays antifungal action against F. oxysporum and confers resistance in soybean against F. oxysporum 45 . Bean pathogen growth can be inhibited by T. harzianum culture filtrate. In Phaseolus vulgaris seeds, Pythium ultimum lessens disease symptoms brought on by this pathogen 46 . The chitinase activity of cell-free culture filtrates improved, and they demonstrated fungus growth inhibition against the pathogens Dematophora necatrix, F. solani, F. oxysporum, and Pythium aphanidermatum. The effect was concentration-dependent, with the maximum growth inhibition rate for all the pathogens tested occurring at a concentration of 25% of the filtrate 28 .
The disease severity (%) dramatically decreased after the application of fungal culture filtrates, and plant growth metrics enhanced. These outcomes are consistent with those attained by Hegazy, et al. 47 in sesame plants and Herrera-Téllez, et al. 48 in tomato plants. Also, inhibition of mycelial growth by cultural filtrates of various bioagents 49 .
Microorganisms can produce certain antimicrobial compounds 50 . There are several opportunities for fungi, especially endophytic fungi, to create secondary metabolites, which are important sources of antimicrobial substances with growth-inhibitory effects on phytopathogens and can be employed as biological control agents 51,52 .
Obviously  www.nature.com/scientificreports/ wilt disease.Furthermore, the application of fungal culture filtrate resulted in an increase in the quantity and width of the fiber layers, as well as the thickness of the cambium region (Ø) in the root and vascular cylinder. This increase was observed in all the analyzed tissues, except for the cortex layers thickness. In addition, the Ø of the stem and epidermis, mesophyll tissue, and the thickness of vascular bundles (V.B.) in leaves were found to be important defenses against FOC infection, and were positively impacted in plants treated with fungal culture filtrate. Observations in plants treated with fungal culture filtrate revealed the presence of a fresh and regenerated vascular bundle. Pre-treating okra seeds with this filtrate led to beneficial changes in their water-conductive elements, facilitating greater absorption of water by the plants and reducing the spread of wilt disease. In fact, the tracheary components of the xylem, which function as the plant's water-conducting system, play a crucial role in supporting plant growth and development 53 . These results are consistent with the study of Ahmed and his colleagues on cucumber plants 54 . Internal root system design can be influenced by a variety of biotic and abiotic stimuli, including bacteria that encourage plant development (PGPM) 55 ; the predominant genera of PGPM are Rhizobia, Acetobacter, Bradyrhizobium, Bacillus, Pseudomonas, Trichoderma 56 , and Penicillium 57 . Plant Growth-Promoting Microorganisms (PGPM) mainly induce changes in the architecture of the internal root system and the structure of root tissues by interfering with the plant's phytohormonal balance pathways that regulate cell origination and differentiation 58 . Several studies have reported that PGPM can provide protection to plants against phytopathogens by inducing the plant's defense mechanisms, which are known as induced systemic resistance (ISR). This phenomenon has been discussed in detail in the review article 59 . As a result of ISR, the cell wall is strengthened by increased lignin production and callose apposition, which slows the spread of phytopathogens across plant tissues 60 . As a result of PGPM-induced changes in root architecture and tissue structure, plants are able to absorb water and nutrients more efficiently, leading to faster overall plant development. Moreover, inoculation with P. chrysogenum and Trichoderma spp. can promote plant growth, enhance biomass accumulation, and improve soil nutrient uptake in plants 59 . A possible defensive mechanism against the okra Fusarium wilt has been suggested by the treatments' increased xylem vessel count and vascular bundle breadth. Our research results are consistent with those reported by El-Sayed 61 ,who demonstrated that using Trichoderma spp. culture filtrates to treat Faba bean plants against wilt pathogens produced strong evidence of resistance in anatomical examinations. When bioinducers are applied to plants through spraying, histopathological changes occur simultaneously with the elicitation of systemic acquired resistance, which is likely to enhance plant growth compared to unsprayed plants.
Overall, the data obtained clearly show that using fungal filtrates, particularly using fungal filtrate of Penicillium as a spray on plants (T6) or overflowed (T8), play a protective defensive function as resistance-inducers against detrimental effects by improving anatomical performance and metabolic defense system. It was obvious that control plants (nematode infection or fungus infection or both together) were negatively internally affected, which might be due to they not having developed mechanisms by which they protected against biotic stresses.

Conclusion
The present study has shown that the bio-control agents gave good control of the disease complex in okra. The application of [Nematode infection (M. javanica) + Fungus infection (F. solani) + Overflowed with fungal culture filtrate (P. chrysogenum)] and [Nematode infection (M. javanica) + Fungus infection (F. solani) + spray with fungal culture filtrate (P. chrysogenum)] were significantly decreased the reproductive factors in the greenhouse and had the largest impact on nematode galling indices on okra roots. [Nematode infection (M. javanica) + Fungus infection (F. solani) + Overflowed with fungal culture filtrate (P. chrysogenum)] was the most effective medication in this regard, reducing illness severity by (28%) comparatively. On the other hand, the treatment [(Fungus infection (F. solani) + (Dovex 50% fungicide with irrigation water)] had the lowest relative illness severity documented (8%) at that time. The findings demonstrated that all examined anatomical properties of okra root, stem, and leaves were lowered by nematode infection, fungus infection, or nematode infection + fungus infection. Therefore, future studies should be focused on the development of economical and efficient mass production of these bio-agents to be used for the management of this disease complex under Egyptian conditions.